Journal: Cells

Article Title: Muramyl Dipeptide Administration Delays Alzheimer’s Disease Physiopathology via NOD2 Receptors

doi: 10.3390/cells11142241

Figure Lengend Snippet: List of antibodies used for the Western blot and immunostaining analyses and all related information, including the name of the company, molecular weight, species, primary and secondary antibody dilution, and targets.

Article Snippet: ABCB1 , Novus biological , 170 kDa , Rabbit , NBP2-67667 , 1/1000 , IgG anti-Rabbit peroxidase , 111-035-144 , 1/2000.

Techniques: Western Blot, Immunostaining, Molecular Weight, Concentration Assay

Journal: Cells

Article Title: Muramyl Dipeptide Administration Delays Alzheimer’s Disease Physiopathology via NOD2 Receptors

doi: 10.3390/cells11142241

Figure Lengend Snippet: The long-term administration of MDP has a different effect on amyloid regarding the sex. Although, MDP induces an increase in the plaque number in the female APP Swe /PS1 treatment with MDP group. ( A ): Unbiased stereological analysis of the 6E10 + plaque number of the male and female APP Swe /PS1 treatment or not with MDP groups in the hippocampus and cortex. Analysis showed a diminution in the plaque number in the male APP Swe /PS1 treatment group in both areas and an augmentation in the Aβ plaque numbers in the female APP Swe /PS1 treatment group in the hippocampus compared to sex-matched APP Swe /PS1 controls. ( B ): Average volume per 6E10 + plaque in µm 3 of the male and female APP Swe /PS1 treatment or not groups with MDP in the hippocampus and cortex. Analysis showed no difference in both sexes and area compared to sex-matched APP Swe /PS1 controls. ( C ): The 6E10+ blood vessel frequency of male and female APP Swe /PS1 treatment or not with MDP groups. Analysis showed an augmentation in the male APP Swe /PS1 treatment group but no difference in the female APP Swe /PS1 treatment groups compared to sex-matched APP Swe /PS1 controls. ( D ): Elisa Aβ40 and Aβ42 of the male and female APP Swe /PS1 treatment or not with MDP groups. Analysis showed an increase in Aβ40 in the male APP Swe /PS1 treatment group but a decrease in the Aβ42 in APP Swe /PS1 treatment group for both sexes compared to sex-matched APP Swe /PS1 controls. ( E ): Ratio of Aβ42/Aβ40 in the male and female APP Swe /PS1 mice treatment or not with MDP groups. Analysis showed a diminution in the male APP Swe /PS1 treatment group but no difference in the female APP Swe /PS1 treatment group compared to sex-matched APP Swe /PS1 controls. ( F ); ( G ): MDP increases the clearance systems through LRP1 and ABCB1 expression in the male group. Western blot analysis of ( F ) LRP1 ( G ) ABCB1 in the male and female wild-type and APP Swe /PS1 treatment or not with MDP groups. Analysis showed an augmentation in LRP1 and ABCB1 expression in the male APP Swe /PS1 treatment group but a diminution in LRP1 and ABCB1 expression in the female APP Swe /PS1 treatment group compared to sex-matched APP Swe /PS1 controls. Data are mean ± SEM ( n = 8 animals/group) * p < 0.05, *** p < 0.001 compared to sex-matched APP Swe /PS1 controls.

Article Snippet: ABCB1 , Novus biological , 170 kDa , Rabbit , NBP2-67667 , 1/1000 , IgG anti-Rabbit peroxidase , 111-035-144 , 1/2000.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot